Fig. 8. Morphologies of GFP-labelled neurons at E14.5 in the cerebral cortex of control and CxCdk5KO mice. GFP immunostaining of coronal sections from CxCdk5KO mice and their littermate controls which were electroporated at E14.5 with CAG-EGFP plasmid. Brains were fixed at E17.5, P1 or P3 and stained with anti-GFP antibody (green). GFP-positive neurons in CxCdk5KO mice were positioned deep down and had multipolar morphology. Scale bars: 100 µm. The bottom-right panel is a higher magnification image of GFP-positive cells in the cerebral cortex of CxCdk5KO mice at P3 obtained with a confocal microscope. Scale bar: 20 µm.