Fig. 3. Development of adipocytes in secondary cultures of quail trunk NCC (TNCC). TNCC that had migrated from cultured thoracic neural tubes were replated into secondary cultures and treated as in Fig. 2. (A) Typical adipocytes showing Oil Red O-stained lipid droplets (left) and SMP-positive glial cells (right) were identified after 20 days in adipogenic conditions. Scale bar: 100 µm. (B) Quantification of TNCC cultures containing adipocytes after treatment with the mentioned media and adipogenic treatments. A total of 42 cultures were analysed.