Fig. 4. Development of adipocytes from the NC during mouse development. Permanent genetic lineage labelling of pre- and post-migratory NC was achieved by crossing transgenic mice carrying a Sox10-Cre construct into a R26-YFP reporter. Double immunolabelling of P28 Sox10-Cre/R26-YFP offspring with anti-GFP (green) and anti-perilipin (red) antibodies was used to identify NC derivatives and adipocytes, respectively. Bisbenzimide was used to identify cell nuclei (blue). Sections show salivary gland and ear (A-D), peri-ovarial (E-H) and trunk subcutaneous (I-L) regions. There is almost complete colocalisation of YFP and perilipin in the salivary gland area, whereas no overlapping can be detected in the ovary and trunk subcutaneous adipose depots. Scale bar: 50 µm.