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Figure 3


Fig. 3. Overexpression of VEGF perturbs skeletogenesis. Sea urchin embryos overexpressing VEGF following microinjection of VEGF mRNA into the egg. (A) WMISH with a Ske-T probe at the gastrula stage. Vegetal view. (B-D) DIC images of pluteus-stage embryos. Arrow, supernumerary elements; circle, abnormal branching. (E-J) WMISH with probes for (E,H) MSP130 at prism stage, (F,I) SM30 and (G,J) MSP130 at pluteus stage.