Fig. 5. EEL-1 interacts with SKN-1 in the yeast two-hybrid assay. (A) The level of two-hybrid interactions between C. elegans EEL-1 and SKN-1. The left column indicates the amino acids of EEL-1 fused to the GAL4 DNA-binding domain, and the central column indicates the amino acids of SKN-1 fused to the GAL4 activation domain. The domain structure of EEL-1 is shown in Fig. 2. SKN-1 has 623 amino acid residues, and its DNA-binding domain comprises residues 549-614. The right column shows the level of growth on selective medium when yeast are transformed with the corresponding plasmids. (B) Two-hybrid interaction between EEL-1 and SKN-1.