Fig. 2. Early gonad development is affected in Cited2 mutant mouse embryos. (A) Gonad development was analysed in wild-type (WT, left panels) and mutant (Mut, right panels) embryos by WISH for Sox9, Cyp11a1 and Sf-1 mRNAs at E11.5 and E13.5. Arrow, gonad; arrowhead, adrenal primordium. (B) Expression of Sox9, Cyp11a1 and Sf-1 was analysed by quantitative real-time RT-PCR on E11.5 microdissected XY gonads from wild-type (black bars) and Cited2 mutant (grey bars) embryos. Results are shown as a percentage of mRNA accumulation in wild-type embryos and represent the mean values obtained with at least four urogenital regions for each genotype (±s.d.); ^*, P<0.05 in Student's t-test.