Fig. 4. Similar mesonephric tubule phenotype in Cited2 and Wt1 mutant mice. (A) Cited2 mutant mouse embryos lack caudal mesonephric tubules. Development of mesonephric tubules was analysed by WISH for Lim1 at E10 (upper panel) and by immunohistochemistry for laminin at E11.5 (lower panel). Caudal mesonephric tubules (arrowheads) are absent from Cited2 mutant (Mut) embryos. (B) Cited2, Wt1 and Sf-1 are coexpressed in the early AGP. Expression of Cited2, Wt1 and Sf-1 was analysed by WISH during urogenital development. The three factors are colocalised in the coelomic epithelium (arrow) and underlying cell layers at E10. Cited2 and Sf-1, but not Wt1, are coexpressed in the adrenal primordium (arrowhead) at E12.5. G, gonad.