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Figure 1


Fig. 1. Expression of Notch target genes of the cHes5 family is repressed by DAPT treatment. (A-F) Stage HH12 embryos cultured for 24 hours in control (A,C,E) or DAPT-containing (B,D,F) medium and processed for whole-mount in situ hybridisation for cHes5.1, cHes5.2 or cHes5.3; dorsal views, with anterior on the left. In control specimens, all cHes5 genes are expressed in the neural tube, and in the anterior (arrows) and posterior (arrowheads) domains of the otic cup, although the expression of cHes5.2 (C) appears fainter and more restricted than that of cHes5.1 (A) and cHes5.3 (E). After 24 hours in DAPT medium, the cHes5 genes are no longer detected in the neural tube or in the otic cup (asterisks in B,D,F). For each Hes gene analysed, the DAPT and control embryos shown were from the same experimental batch.