Fig. 5. Basal localization of vimentin is dispersed in mitosis, whereas transitin persists in the cell cortex. Blue is DAPI nuclear staining. Basal is up. (A) Colocalization of vimentin (green) and transitin (purple) in radial fibers of neuroepithelium (NE) cells (arrows) and in the basal cortex of mitotic NE cells (arrowheads) is observed. (B) Non-filamentous vimentin staining (green) is observed basally in prometaphase, but dissociated from the cortex in ana- and telophase. (C,D) Phosphorylated vimentin is found abundantly in early M phase. Transitin persists in the cortex throughout M phase (lower panels). In the late phase of mitosis, transitin is preferentially segregated in one of the two daughter cells, despite the cell cleavage plane being parallel to the apicobasal axis.