Fig. 2. Rbpsuh is required for gliogenesis in sensory ganglia. (A-D) Transverse sections of dorsal root ganglia from control and Wnt1-Cre⁺ Rbpsuh^fl/fl mouse embryos at E14.5 (A,B) and E18.5 (C,D) were stained for neurons (TuJ1⁺, green) and glia (BFABP⁺, red). Scale bar: 50 µm. (E) There was no difference between control and Wnt1-Cre⁺ Rbpsuh^fl/fl embryos at E10.5 in terms of the number of p75⁺ neural crest progenitors that initially migrated into the ganglion (three sections per mouse and three mice per genotype). (F) Although no difference in neurogenesis was observed at E10.5 (glia could not be detected at this stage), neurogenesis was significantly (^*, P<0.05) reduced in Wnt1-Cre⁺ Rbpsuh^fl/fl sensory ganglia at E14.5 and E18.5 (four to nine sections per mouse, seven to eight mice per genotype). Gliogenesis was even more profoundly reduced, as virtually no glia could be detected in Wnt1-Cre⁺ Rbpsuh^fl/fl sensory ganglia. (G,H) We did not detect any difference in the rate of proliferation (G, the percentage of cells that incorporated a 30-minute pulse of BrdU in vivo at E14.5), or cell death (H, the percentage of cells expressing activated caspase 3). Error bars indicate s.d.