Fig. 4. Rbpsuh deletion leads to profound defects in gliogenesis despite normal neurogenesis in sympathetic ganglia. (A,B) Transverse sections of the sympathetic chain from control or Wnt1-Cre⁺ Rbpsuh^fl/fl mouse embryos at E13.5 (A) or E18.5 (B) were stained for neurons (TuJ1⁺, green) and glia (BFABP⁺, red). Scale bar: 20 µm. (C) The number of migratory neural crest cells (p75⁺) in the sympathetic ganglion of the control and Wnt1-Cre⁺ Rbpsuh^fl/fl embryos did not differ at E10.5. (D) The numbers of neurons (TuJ1⁺) and glia (BFABP⁺) per section through the E10.5 (three mice per genotype), E14.5 (four to seven mice per genotype) and E18.5 (four to seven mice per genotype) sympathetic chain. Numbers of BFABP⁺ glia in the Wnt1-Cre+ Rbpsuh^fl/fl sympathetic chain were significantly reduced relative to control (P<0.05). (E,F) We did not detect any difference in the rates of proliferation (E, the percentage of cells that incorporated a 30-minute pulse of BrdU in vivo at E14.5) or cell death (F, the percentage of cells expressing activated caspase 3) (three mice per genotype). For all developmental stages, between five and twelve sections were counted per mouse at upper and lower thoracic levels. Error bars represent s.d.; ^*, P<0.05.