Fig. 2. RGA-2 colocalises with actin microfilaments in the epidermis. (A-C) Confocal lateral images of C. elegans embryos carrying the rescuing rga-2::gfp fusion F1 (see Fig. 3). (A) 1.2-fold, (B) 1.5-fold and (C) 3-fold stage embryos. GFP expression was restricted to the epidermis; it initially highlighted cell-cell junctions (white arrows), plus thin and short filaments that progressively became organised into a circumferential filamentous network. (D) Confocal image of a small area of the body from a rga-2::gfp transgenic 3-fold embryo showing GFP fluorescence (green, left), bodipy-phallacidin staining to visualise actin (red, middle) and merged picture (right) showing colocalisation. Arrows, actin in underlying muscles; arrowheads, epidermal cell-cell junctions. (E-J) DIC (top) and GFP fluorescence (bottom) pictures of adults, illustrating expression in sensory support cells of the head (E,F), vulval cells (G,H) and rectal cells (I,J), which are epithelial. Scale bars: 10 µm.