Fig. 4. Severe pulling on junctional complexes in rga-2 and mel-11 mutants. (A) Still projections from time-lapse videos of wild-type (top) and rga-2(hd102) (bottom) C. elegans embryos containing the mcIs46[dlg-1::rfp] transgene. Time is shown in seconds (bottom right corner). Insets in the rightmost images show single focal plane enlargements of boxed regions. For original wild-type and rga-2 videos, see Movies 5 and 6 in the supplementary material. (B) Additional still images for wild-type, mel-11(it26), rga-2(hd102) embryos and embryos laid by mel-11(it26) mothers after injecting rga-2(RNAi). Arrows, indentations; arrowheads, deformed seam cells. (C) Summary of the defects observed in rga-2 and mel-11 mutants. (D) The number of indentations (from videos such as in A) that were perpendicular to the plane of junctions and at least twice the width of a junction. Counting was performed only in the head (hyp6/hyp7 and H1/H2 area) on at least three embryos per genotype.