Fig. 5. The GAP domain of RGA-2 enhances GTP hydrolysis by RHO-1 and CDC-42. Results of GAP assays obtained by incubating purified GTPases and GST-RGA-2_GAP fusion protein in the presence of [³²P]GTP (see Materials and methods), resolving the reaction products by thin layer chromatography (see Fig. S3 in the supplementary material), and quantifying the GTP/GDP fraction with a phosphorimager. Each time point represents the fraction of GTP hydrolysed after 2 minutes as a function of RGA-2_GAP concentration. The graph is representative of four independent experiments.