Fig. 5. Characterization of Pdx1-Nkx6.2 transgenic mice. (A) The Pdx1-Nkx6.2 transgene contains the Pdx1 promoter fused to Nkx6.2 cDNA followed by an IRES-lacZ-polyA cassette. (B) Mirroring the Pdx1⁺ domain, lacZ expression in Pdx1-Nkx6.2 mice is detected in the ventral (vb) and dorsal (db) pancreas anlagen at E10.5. (C-H) In Pdx1-Nkx6.2 mice, expression of ß-galactosidase (ß-gal, red) and Pdx1 (green) overlaps completely at E14.5 (C-E), whereas a subset of Pdx1⁺ cells fails to express ß-gal at E18.5 (F-H, arrows in H). db/vb, dorsal/ventral pancreatic anlagen Scale bars: 50 µm.