Fig. 6. Ftm is located at the basal body and is necessary for normal cilia numbers in mice. (A) Scanning electron microscopy images at E7.5. In the wild-type node (left), each cell possesses a single cilium on its surface, whereas in Ftm^-/- embryos (right), the number of cilia is reduced and the cilia are malformed. (B) Immunohistochemistry on sections of limb mesenchyme at E11.5. In the wild type (left), each mesenchymal cell exhibits cilia stained by acetylated -tubulin antibody. By contrast, in Ftm^-/- embryos (right), the number of cilia is reduced. (C) Immunostainings on MEFs. (i) Ftm (red) is located at cilia, but does not overlap with acetylated -tubulin (green). (ii) Ftm (red) is co-localized with -tubulin (green) in the basal body of the cilia. The blue staining marks the nuclei by DAPI.