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Figure 1


Fig. 1. Angiogenic cells are detected in wing somatopleure before muscle progenitor cells in avian embryos. (A-F) Adjacent transverse sections of chick embryos, at the wing level, at 19 (A,D), 25 (B,E) and 30 (C,F) -somite stages were hybridized with Vegfr2 (A-C) and Pax3 (D-F) probes. (G-I) Transverse sections of quail embryos, at the wing level, at 22 (G), 24 (H) and 32 (I) -somite stages were hybridized with Pax3 probe (blue) and then incubated with QH1 antibody (brown). Arrowheads point to endothelial progenitor cells visualized with Vegfr2 probe (A,B) or QH1 antibody (G,H). Arrows indicate the overlapping domain of expression of Vegfr2 (A) and Pax3 (D) in somites. NT, neural tube; Nc, notochord; So, somites.