Fig. 8. Co-treatment of recombinant BMP15 and FGF8B promoted expression of Pfkp and Ldha mRNA and glycolytic activity in OOX cumulus cells. OOX cumulus cells were treated with recombinant FGF8B (0, 10 or 100 ng/ml) with or without either BMP15 (500 ng/ml) or GDF9 (500 ng/ml), or co-cultured with oocytes (2 oocytes/µl), and (A) expression levels of Pfkp and Ldha mRNA and (B) glycolytic activity in OOX cumulus cells were examined. Mean±s.e.m. The values indicated by different letters (a, b and c) are significantly different (P<0.05).