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Figure 4


Fig. 4. Molecular characterization of P-element insertion lines. (A) The positions of P-element insertions analyzed and the primers that were used [DMCYLDF3 (DCYF3), DMCYLDR6 (DCYR6), DMCYLDF7 (DCYF7) and DMCYLDR9 (DCYR9)] are shown relative to the five transcripts of Drosophila CYLD that have been identified. (B) The P-element-insertion lines f00135, f00814, f02492 and d10472 were analyzed by reverse transcriptase (RT)-PCR for CYLD mRNA. The results were compared with wild-type flies (w1118). rp49 (also known as RpL32) RNA was used as a control for the amount of cDNA used in the RT-PCR reaction (lower panel).