Fig. 2. The density of cortical actin depends upon the level of expression of C-cadherin at the cell surface. (A-C) Pairs of images from double-stained (C-cadherin, red; F-actin, green) animal caps. (A) Control levels of C-cadherin and cortical actin. (B) An animal cap from a Xenopus embryo depleted of C-cadherin by antisense oligo injection into oocytes. (C) An animal cap from an embryo overexpressing C-cadherin. Cortical actin is increased when C-cadherin is overexpressed and decreased when C-cadherin is depleted. (D) Quantitation of the cortical actin by measurement of pixel intensity. Scale bars: 10 µm.