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Figure 3


Fig. 3. Effects of C-cadherin mutants on cortical actin assembly. (A) Animal caps dissected from Xenopus embryos at stage 9 were fixed with 2% TCA for anti-C-cad immunostaining (upper row) or with FG fixative for F-actin staining (lower row). Pairs of images are shown from embryos that were untreated (Control, left), or injected with C-cad G-A (does not bind p120 catenin, center) or with C-cad {Delta}CBD (does not bind ß-catenin, right). Both cadherin mutant mRNAs are expressed (see also Fig. 4). However, neither causes a significant increase in cortical actin, and the C-cad G-A mutant caused loss of cortical actin. (B) F-actin levels quantitated by pixel intensity. *, P<0.05. Scale bars: 20 µm.