Fig. 7. Overexpression of an Xflop mutant (R112A) that lacks G-protein-coupling activity does not increase cortical actin assembly. (A) Alexa 488-conjugated phalloidin staining showing that overexpression of the R112A mutant (1 ng mRNA injected) does not mimic the capacity of wild-type Xflop to increase cortical actin assembly. (B) Quantitation by pixel intensity shows that R112A had no effect on cortical actin assembly. ^*, P<0.01. Scale bar: 50 µm.