Fig. 8. Depletion of Xflop or LPA receptors causes reduced C-cadherin expression and reduced cortical actin assembly that can be rescued by C-cadherin mRNA. (A-C') Pairs of images (left, C-cadherin staining; right, F-actin staining) from Xenopus embryos that were untreated (A), Xflop-depleted (B), or LPA1 and LPA2-depleted (C). Both cadherin staining and cortical actin assembly are reduced by Xflop and LPA1/2 depletion. The effects of each depletion can be rescued by subsequent injection of C-cadherin mRNA (B',C'). (D,E) Cortical actin levels were quantitated by pixel intensity. ^*, P<0.01. Insets are western blots showing that depletion of Xflop (D) or LPA1/2 (E) receptors also reduced the total levels of C-cadherin. tubulin was used as a loading control. Scale bars: 10 µm.