Fig. 5. Synergistic interaction between ful-1 and as1 alleles. (A-D) Bright-field stereomicroscope images (A,B) and SEMs (C,D) of stage 17 fruits. The ful-1 mutant exhibits small valves and enlarged and creased repla (A,C). The as1-104 allele enhances the mutant phenotype of ful-1, so that as1-104 ful-1 double mutants show very small valves and very compressed and distorted repla (B,D). (E-G) GUS staining in cross sections of fruits containing the ful-1 reporter. The reporter is expressed only in the valves of as1-104 (E), ful-1 (F) and as1-104 ful-1 (G) fruits. Note the very reduced width of valves and the large size of repla in the double mutant (G). The as1-104 fruit in E is heterozygous for the ful-1 reporter. (H,I) Stage 17 fruits from the as1-1 ful-1 rpl-1 triple mutant showing a reduced mutant phenotype compared with the as1-104 ful-1 double mutant. SEM (H) and transverse section (I) displaying GUS staining from the ful-1 reporter in the aberrant valves. All fruits are in the er background. Scale bars: 1 mm in A,B; 400 µm in C,D,H; 200 µm in E-G,I.