Fig. 4. Expression of Ahd2 is decreased in SNc mdDA neurons of Pitx3^+/- mice. (A) Quantitative in situ hybridization analysis of the Ahd2, Th, Vmat2 and a-synuclein mRNA expression levels in the substantia nigra pars compacta (SNc) of Pitx3^+/+ (wt, white bars, n=8) and Pitx3^+/- (black bars, n=5) mice. The graphs represent the average labeling expressed as percentage of levels in Pitx3^+/+ mice ±s.e.m. and were analyzed by using the Student's t-test (^*, P<0.05). (B) Typical examples of individual autoradiographic film labeling from coronal sections through the meso-diencephalic dopaminergic (mdDA) area containing the SNc from adult wild-type mice, which were used for determination of mRNA levels.