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Figure 3


Fig. 3. GFP::SYS-1 nuclear asymmetry between A-P sisters is Wnt signal- and proteasome-dependent. Embryos are shown at the 2MS/2E stage, with lines connecting the MSa/MSp and Ea/Ep sisters. (A) Immunofluorescence micrographs of GFP::SYS-1 in various mutant backgrounds (as indicated to the left). Left column, anti-GFP; right column, DAPI. (B) Independent regulation of GFP::SYS-1 (a,d,g) and POP-1 (b,e,h) asymmetry. (a-c) Wild type; (d-f) mom-5(or57) showing symmetric GFP::SYS-1 and asymmetric POP-1; (g-i) wrm-1(RNAi) showing asymmetric GFP::SYS-1 and symmetric POP-1. (C) Wild-type (a-c) and rpn-8(RNAi) (d-f) embryos double stained with antibodies to GFP and PIE-1. Arrow, germline precursor; arrowheads, somatic nuclei that stain weakly with the PIE-1 antibody. Scale bar: 10 µm.