Fig. 5. Dichaete is co-expressed and co-regulated with SoxN and is required for denticle formation. (A) Double-staining of wild-type (wt) stage-15 embryos with anti-SoxN (green) and anti-Dichaete (anti-D; red); notice the complete overlap between SoxN and Dichaete expression (yellow). (B) Staining of stage-15 wg^cx4 mutant embryos with anti-D; notice that Dichaete is ectopically expressed. (C) Staining of stage-15 armVP16Gal4-UAS-Egfr^DN embryos with anti-D; notice the reduction in Dichaete expression. (D) Wild-type cuticle. (E) SoxN^U6-35 mutant cuticle. (F) SoxN^U6-35;D/+ mutant cuticle.