Fig. 6. SoxN and Dichaete negatively regulate Wg pathway activity. (A-D) Anti-En stainings of late-stage-10 embryos. Insets show higher magnifications of the En expression around the ventral midline. Black lines indicate the ventral midline. (A) Wild type (wt). (B) armVP16Gal4-UAS-SoxN; notice the reduction in En expression. (C) SoxN^U6-35; notice the increase in En expression. (D) SoxN^U6-35;D/+; notice that the increase in En expression is stronger than in SoxN single mutants. Cartoon: regulatory interactions between the Der- and Wg-pathway activities, SoxN, Dichaete (denoted by D) and svb. SoxN and Dichaete expression is stimulated by Der- and repressed by Wg-pathway activity. SoxN and Dichaete negatively regulate Wg pathway activity and activate the expression of svb. svb is not required for the establishment of the epidermal SoxN and Dichaete expression domains. However, svb is necessary for the maintenance of SoxN and Dichaete expression during the late stages of embryogenesis.