Fig. 3. bri1 mutation leads to an elevation of FLC mRNA levels in FRI and in autonomous mutants. Absolute (A) and relative (B) FLC expression in bri1-201 ld-3 double mutants, compared to the respective single mutants, under long-day photoperiods. Samples were taken at the time indicated (number of days of growth), until the flower buds were visible. (C) FLC expression in bri1-201 ld-3 and bri1-202 ld-3 double mutants and in the respective single mutants after 14 and 30 days of growth under short days. (D) FLC mRNA levels in 30-day-old bri1 ld double-mutant combinations, and (E) in the double-mutant bri1 fca, bri1 FRI and bri1 gi lines under long days. FLC mRNA abundance was monitored by RNA-blot analysis. (A-E) The ACTIN 1 (ACT1) gene was used as a loading control. FLC levels were quantified, values were normalized to the levels of ACTIN 1, and the highest value in each separate experiment was set to be 1.