Fig. 4. ofs spermatocytes activate an abnormal Cyclin A pattern due overexpression of Rux. Whole-mount immunofluorescent images of siblings from a single cross. (A,B) Rux⁺ progeny show nuclear Cyclin A (CycA, green) co-localized with Rux (red) accumulation in ofs mutants (B, arrow), but not in their heterozygous siblings (A, arrowhead). (C) Siblings heterozygous for ofs still show Cyclin A nuclear translocation in the absence of rux function (arrowhead). (D) ofs mutants inheriting the rux⁹ mutation have only cytoplasmic localization of Cyclin A (arrow).