Fig. 5. ofs encodes the main eIF4G in spermatocytes. (A) Map of the genomic sequence of a portion of the right arm of Chromosome 3 indicating breakpoints of informative deletions (dark gray) and the Z3-3283 point mutation relative to transcripts (red, oriented 5' to 3') and coding regions (blue) for both CG10192 (Ofs) and CG33111. Also shown: in situ probes (light gray), insertion sites for nearby transposable elements (red circles) and flanking genes (green). (B) Predicted Ofs protein and Drosophila eIF4G. Locations are noted for the alternate splice (red), the premature stop in Z3-3283 (asterisk), predicted eIF4E-binding sites (green) and homologous regions (gray). Conserved domains are 27% identical and 43% similar, and include the predicted eIF4A-binding domains. (C-D') In situ hybridizations to wild-type (wt) testes. Tips are marked with asterisks. (C) Antisense probe for ofs (see panel A) showed expression in spermatocytes. (D) Antisense probe to eIF4G showed low expression at the testis tip, and was undetectable thereafter. By contrast, somatic tissue stained strongly (arrows). (C',D') Sense probe controls.