Fig. 2. Loss-of-function mutations in eIF4G2 result in male sterility. (A) Diagram of eIF4G2 intron/exon structure and four strong loss-of-function alleles. Gray, protein coding sequence; black, untranslated regions; diagonal lines, region encoding conserved middle domain. (B) Apical third of a wild-type testis. (C) Apical third of a testis from an eIF4G2 homozygote. (D) Part of testis from eIF4G2 homozygote. (E) Part of testis from eIF4G2 homozygote carrying a single copy of the genomic rescue transgene. eIF4G2 mutant flies were eIF4G2^BR21-37/eIF4G2^Z3-3283. Scale bars: 100 µm.