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Figure 5


Fig. 5. eIF4G2 is required for translation of twine and cycB in spermatocytes. (A,B) Anti-CycB immunofluorescence on (A) wild-type and (B) eIF4G2 mutant testes. Bracket indicates expression of CycB in mature spermatocytes in wild type. (C,D) X-gal staining of (C) wild-type and (D) eIF4G2 mutant testes expressing a twine-lacZ reporter. (E,F) In situ hybridization on wild-type (E) and eIF4G2 (F) testes with cycB antisense probe. (G,H) In situ hybridization on wild-type (G) and eIF4G2 (H) testes with twine antisense probe. Scale bars: 100 µm. eIF4G2 mutant flies were eIF4G2BR21-37/eIF4G2Z3-3283.