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Figure 6


Fig. 6. eIF4G2 is required for proper spermatid differentiation.Live squashes of wild-type and mutant germ cells initiating spermatid differentiation. (A,C,E,G,I,K) Phase contrast. (B,D,F,H,J,L) Hoechst staining. (A,B) Wild-type early round spermatids with haploid nuclei (arrow) and mitochondrial derivative (arrowhead). (C,D) Elongating spermatids in wild type. (E,F) Late-stage eIF4G2 cells with large nuclei (arrow) and aggregating mitochondria (arrowhead). (G,H) Terminal cells in eIF4G2 with aggregated mitochondria (arrowhead), large nuclei (arrow) and aberrant partial cellular elongation. (I,J) twine early spermatids. (K,L) twine elongating spermatids. All images are at the same magnification. Scale bar: 20 µm. eIF4G2 mutant flies were eIF4G2BR21-37/eIF4G2Z3-3283. twine mutant flies were tweHB5/tweK08310.