Fig. 1. Generation of Evc^-/- mice. (A) Wild-type Evc allele and the targeting vector. The length of homologous sequence flanking the lacZ and neomycin resistance cassettes in the targeting vector is indicated; the two loxP sites are shown as triangles. (B) Southern blot analysis confirming homologous recombination at the Evc locus. DNA from wild-type and Evc^+/- mice was digested with HindIII (left) or EcoRI (right) and hybridised, respectively, with probes 1 and 2 (see A). Asterisks designate hybridisation signals corresponding to the targeted allele. (C) PCR products from tail-tip genotyping. (D) RT-PCR showing total absence of Evc transcript in Evc^-/- mice and unaffected transcription of Crmp1. Three different primer pairs were used to amplify Evc cDNA (NM_021292) downstream of exon1 and Crmp1 cDNA (NM_007765). Hprt was used as a control.