Development -- Ruiz-Perez et al. 134 (16): 2903 Figure IG2

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Fig. 2. Evc localises to the basal body and is not required for ciliogenesis. (A) Acetylated tubulin and ${gamma}$ -tubulin immunofluorescence (green) distinguish the ciliary axoneme and the two centrioles, respectively, in mIMCD3 cells. Anti-Evc (red) reveals Evc to be localised at the base of the cilium. Arrowheads, centrioles. (B) ${gamma}$ -tubulin (green) and Evc (red) immunofluorescence show that Evc signal overlaps with one of the two centrioles. (C) In situ Evc immunostaining of wild-type and Evc^-/- chondrocytes of P0 proximal tibia demonstrate Evc localisation, the presence of cilia in the mutant and the specificity of the antibody. Scale bar: 2 µm.