Fig. 1. General phenotypes of the Egfl7-knockout mouse. (A,B) Egfl7^+/- (A) and Egfl7^-/- (B) E14.5 littermates. Arrowhead indicates systemic oedema in the Egfl7^-/- mouse. (C,D) CD31 [also known as PECAM1; brown, endothelial cell (EC) marker] and Hematoxylin (blue, H, nuclear marker) staining on transverse sections of the neck dermis from Egfl7^+/+ (C) and Egfl7^-/- (D) E14.5 littermates. Red bars indicate the thickness of the epidermis. Increased thickness in the Egfl7^-/- tissue is a hallmark of oedema. (E) Body weights of multiple pairs of Egfl7^+/+ and Egfl7^-/- littermates at the indicated ages. Adults are all between 4-7 months. P values are calculated with unpaired, two-tailed t-test with 95% confidence interval (the same method is used for all P values unless specified otherwise). (F-M) Hypoxyprobe (brown) and Hematoxylin (blue) staining on sections of adult organs from Egfl7^+/+ and Egfl7^-/- littermates. Genotype and organ names are indicated. Arrowheads indicate example hypoxic cells. The actual size represented by the width of the panel: 8.5 mm (A,B); 0.87 mm (C,D); 430 µm (F-M).