Fig. 2. Loss of Egfl7 results in delay of vascularization. (A-D) CD31 wholemount staining on embryonic mouse hearts from Egfl7^+/+ (A,B) and Egfl7^-/- (C,D) littermates at E12.5 (A,C, dorsal view of the left ventricles) and E14.5 (B,D, lateral view of the left ventricles, dorsal is to the right). Asterisks, areas without coronary vasculature; weak signal is from the underlying endocardium. (E,F) CD31 wholemount staining on embryonic heads from Egfl7^+/+ (E) and Egfl7^-/- (F) littermates at E10.5. Open arrowheads, major cranial vessels. (G) Ventral coronary vascular coverage of multiple pairs of Egfl7^+/+ and Egfl7^-/- littermates at E12.5 and E14.5. Horizontal bars represent means. Because of large inter-litter variation at E12.5, littermate relationship is specified with numbers in the graph. Significant difference is marked with an asterisk next to each P value. (H) Major cranial vessel counts of multiple pairs of Egfl7^+/+ and Egfl7^-/- E10.5 littermates from two knockout lines. ins, insertional knockout line; hom, homologous recombination knockout line. (I-N) Isolectin B4 (EC marker) staining on flatmount neonatal retinas from Egfl7^+/+ (I-K) and Egfl7^-/- (L-N) littermates at P2 (I,L), P5 (J,M) and P8 (K,N). (O,P) Retinal vascular coverage (O) and size (P) of multiple pairs of Egfl7^+/+ and Egfl7^-/- newborn littermates at the indicated ages. Solid red arrowheads indicate example aberrant EC clusters; arrows indicate sprouts at the retinal vascular migration front. The actual size represented by the width of the panel: 0.9 mm (A,C); 1.4 mm (B,D); 3.6 mm (E,F); 3 mm (I,J,L,M); 4.7 mm (K,N); K and N are assembled from overlapping 4x images.