Fig. 4. EGFL7 regulates the collective migration of ECs, but not the movement of individual ECs. (A,B) Live imaging at days 4-7 of cultured mouse aortic rings isolated from Egfl7^+/+ (A) and Egfl7^-/- (B) littermates. Colored arrowheads track individual sprouts over time. (C,D) Quantification of sprout length (C) and sprout extension velocity (D) on cultured aortic rings isolated from the Egfl7^+/+ (blue bars) and Egfl7^-/- (red bars) littermates. (E) Transwell migration assay performed on fibronectin- or EGFL7-coated inserts using ECs isolated from four pairs Egfl7^+/+ and Egfl7^-/- littermates. Migration efficiency was compared between Egfl7^-/- (ko) and Egfl7^+/+ (wt) samples and the ratios plotted. (F) Migration velocity of individual HUVECs on fibronectin (FN), EGFL7, or combined substrate. The actual size represented by the width of the panel: 1.09 mm (A); 0.73 mm (B).