Fig. 7. FGF-induced SMG branching morphogenesis is independent of Npn1 function in mice. (A) Semi-quantitative RT-PCR analysis indicated that the expression of Fgf10, Fgf7 and Fgfr2 in the SMG explants were not altered when the SMG cultures were treated with Npn1 antisense ODNs. Gapdh is an internal control. C, no treatment; S, SMG cultures treated with Npn1 sense ODNs. (B) Fgf7 (500 ng/ml) and Fgf10 (1000 ng/ml) still effectively promoted SMG branching formation in the presence of Npn1 antisense ODNs (a). Representative photographs were taken from 24-hour cultures. A summary of five independent experiments is shown in b. Scale bar: 100 µm. AS, treated with Npn1 antisense ODNs; C, control peptide; S, treated with Npn1 sense ODNs.