Fig. 8. Sema3A restricts migration of cultured SMG epithelial cells. (A) The migration of mouse epithelial cells in mesenchyme-free SMG explant culture was restricted by synthetic Sema3A peptide (100 ng/ml). (B) The extent of epithelial cell migration is summarized at three time points, as indicated. The migration ratios were obtained from dividing the area measured at each time point by the area measured at 0 hours. (C) Sema3A caused cytoplasmic constriction of the SMG epithelial cells. Dissociated epithelial cells from E13.5 SMG were cultured for 2 days and then treated with synthetic Sema3A or scrambled peptide for another 1 or 2 days. Cells were fixed with paraformaldehyde and stained with rhodamine phalloidin. Scale bar: 100 µm.