Fig. 2. Inactivation of TAF4 in adult epidermis. (A) Ethidium bromide-stained agarose gel of products of a triplex PCR. The locations of the floxed and deleted fragments amplified from the dermis (D) or epidermis (E) 2 weeks after oil or Tam injection are shown. (B) Immunostaining of skin biopsies 2 weeks after oil (above) or Tam (below) injection with anti-TAF4 antibody. Abbreviations as described in Fig. 1. (C) Immunostaining of skin biopsies 2 weeks after oil or Tam injection with anti-Ki-67 antibody. Arrows indicate representative positive staining nuclei. (D) Immunostaining of skin biopsies with anti-keratin 6 antibody. The arrows indicate the interfollicular epidermis. (E) Histological analysis of representative Haematoxylin-Eosin-stained paraffin sections from dorsal or ventral regions of oil- or Tam-injected mice. Hair follicles (HF) are indicated and arrows indicate the location of the basal membrane. Scale bar: 25 µm in D; 50 µm in E.