Fig. 2. Cross sections of lhw roots and hypocotyls. (A) Schematic of stele in cross sections from wild type (WT), lhw and wol-1; pericycle is in blue and xylem in yellow; wild type and lhw were traced from 2F and 2G, respectively. (B-G) Bright-field images of wild-type and lhw root sections taken at increasing distance from the tip of the root; for each, the same pericycle cell is marked with a blue star for orientation: (B) wild type, 30 µm; (C) lhw-2, 30 µm; (D) wild type, 60 µm; (E) lhw-2, 60 µm; (F) wild type, 120 µm; (G) lhw-2, 120 µm. (H,I) Toluidine-blue staining of wild type (H) and lhw-2 (I) in the mature zone. (J,K) Section through the lower third of the hypocotyl in wild type (J) and lhw-2 (K). (L) Center of a wol-1 root, 66 µm from the tip. (M) Center of a wol-1;lhw-1 root, 66 µm from the tip. (N,O) DIC (N) and toluidine-blue (O) images of wol-1;lhw-1 mature roots, showing the very reduced stele filled with xylem elements. Each image pair is at the same magnification. Scale bars: 20 µm.