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Figure 3


Fig. 3. Measures of auxin response in lhw. (A-C) Confocal images of wild type (WT, A) and lhw-1 (B,C) expressing J0121::GFP (green). (A,B) After treatment with 20 µM NPA for 7 days. (C) After treatment with 30 nM 2,4D for 7 days. (D,E) DIC images of roots treated with 20 µM NPA for 21 days. Black arrows point to xylem elements. (F-I) Bright-field images of 7-dpg seedling root tips; (F,G) DR5::GUS expression; (H,I) PIN4::GUS expression. Each image pair (and A-C) is at the same magnification. Scale bars: 20 µm.