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Figure 5


Fig. 5. TGFß1 disrupts Fgf10 expression in the mouse foregut mesoderm. (A,B) X-Gal staining of RARElacZ foregut cultured in TGFß1-containing medium reveals strong RARElacZ signal at 3 hours (A) and 72 hours (B) in culture. (C,D) Fgf10 is expressed in control foreguts (C, arrowheads) and is suppressed in TGFß1-treated foreguts (D, asterisk). (E) Real-time PCR results showing the downregulation of Fgf10 in MLg cells by TGFß1 treatment (*, P<0.05 by Student's t-test). (F,G) FGF10-but not PBS-soaked heparin bead rescues Nkx2.1 expression and bud outgrowth in TGFß1-treated foreguts (G, arrowhead). Ht, heart; Lu, lung; Th, thyroid; Ctr, control. Scale bar: 250 µm in F.