Fig. 2. Rescue of Drosophila wing development and vg reporter expression in ap⁰ discs by UAS-N^intra and UAS-N^ECN clones. Tub1-Gal4/UAS-N^intra (A) and Tub1-Gal4/UAS-N^ECN (B-E) clones monitored by autonomous expression of Wg (red in A,C), UAS-GFP (green in B), BE-vg^GFP (green in C,D), or BE-lacZ (blue in E), induce expression of both QE reporter genes (1XQE-lacZ, blue in A,C; 5XQE-DsRed, red in B,E), as well as rn-lacZ (blue in B) in surrounding cells (note that rn-lacZ expression extends beyond that of 5XQE-DsRed). Restored growth of the wing pouch and surrounding rn-only territory is indicated by the greatly expanded inner ring of Wg expression (A,C; compare with Fig. 1E,F). Clones induced in early (D) or mid- (E) third instar also induce non-autonomous QE reporter expression, although the range of the response is greater for clones induced during the first instar (A-C). Here, and in the remaining figures, clones were induced during the first larval instar (except for D,E), and the clone genotypes are indicated by colored ovals (representing presence of marker expression within the respective clones), as indicated in each experiment.