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Figure 3


Fig. 3. Rescue of Drosophila wing development and QE reporter expression by UAS-vg clones. (A-E) Tub{alpha}1-Gal4/UAS-vg clones [marked by UAS-GFP (green in A,C-E), or the absence of GFP (black in B); clone genotype indicated by colored outline with black filling representing absence of marker expression within the clone] induce Vg (blue in A) and 1XQE-lacZ (red in A,C-E) in surrounding cells in ap0 discs. Tub{alpha}1-Gal4/UAS-vg clones ectopically express wg (wg-lacZ, blue in B, white arrows) and their ability to induce 1XQE-lacZ in surrounding cells depends on the Wg they secrete. Tub-Gal4/UAS-vg clones that are also wg0 fail to do so (C), unless they co-express exogenous Wg (D), or membrane-tethered Wg (Nrt-Wg, E), in which case they do so at long range, or only in adjacent cells, respectively. Coincident GFP and 1XQE-lacZ expression appear yellow in C,E, and white when co-stained for Wg (blue) in D.