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Figure 5


Fig. 5. Tub{alpha}1>vg clones act at short range to induce vg and QE reporter expression in neighboring UAS-Nrt-wg clones. (A-D) ap0 discs containing Tub{alpha}1>vg clones (black by absence of DsRed, left column), C765-Gal4/UAS-Nrt-wg clones (yellow by coincident expression of Flu-tagged Nrt-Wg, green, and DsRed), and `double' Tub{alpha}1>vg C765-Gal4/UAS-Nrt-wg clones (green by expression of Nrt-Wg in the absence of DsRed) are shown stained for Vg (A,C) or 1XQE-lacZ (B,D) expression (blue). (A,B) Double clones (green) within the prospective Drosophila wing pouch (A,B, 2) show peak expression of Vg (bright blue) and 1XQE-lacZ and induce peak expression in adjacent cells outside of the clone (note that the green territories are nested inside the larger bright blue territories, right column). Tub{alpha}1>vg clones (A, black, 1) show only moderate Vg expression (derived only from Tub{alpha}1>vg, dull blue). (C,D) Tub{alpha}1>vg clones (1, black) that abut C765-Gal4/UAS-Nrt-wg clones (3, yellow) induce cells in the latter to express peak levels of Vg and 1XQE-lacZ. The induction is not limited to those C765-Gal4/UAS-Nrt-wg cells that abut the Tub{alpha}1>vg clone, but propagates over many cell diameters into the C765-Gal4/UAS-Nrt-wg clone. Vg and 1XQE-lacZ expression are also upregulated in the Tub{alpha}1>vg cells that abut the C765-Gal4/UAS-Nrt-wg clone.