Fig. 3. 5XQE transgene expression requires Wg input. (A) 5XQE>CD2>vg expression (CD2, red) in a wild-type wing disc (the D-V boundary is marked by BE-vg^GFP expression, green). (B,C) Rescue of wing development in vg⁰ 5XQE>vg clones [black by the absence of both GFP and CD2, and blue by the expression of 1XQE-lacZ (B) or Vg (C); vg⁺ 5XQE>CD2>vg tissue appears yellow]. Note in C that the level of Vg expressed within the vg⁰ 5XQE>vg clone (outlined in green) is similar to that in neighboring wild-type tissue (vg⁺ 5XQE>CD2>vg, outlined in red). (D) Small, late-induced arr⁰ clones (black by absence of DsRed, green; white arrows) in a 5XQE>CD2>vg wing disc composed almost entirely of large, early-induced 5XQE>vg clones (remaining patches of arr⁺ 5XQE>CD2>vg-expressing tissue are marked by CD2 expression, red). 5XQE>vg (Vg, blue) is reduced or absent in the arr⁰ clones. (E) 5XQE-DsRed expression (blue) is lost in an arr⁰ clone located inside a Tub1>vg clone (black arrow; black by the absence of both CD2, green, and GFP, red), and in arr⁰ clones in the surrounding Tub1>GFP>vg tissue (white arrows; appears red).