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Figure 3


Fig. 3. A CMV-endoglin transgene rescues hemangioblast activity in Eng-/- ES cells. (A) FACS analysis for endoglin expression in Eng+/-, Eng-/- and -/-:R5 (rescued clone) ES cells. Upper panels, undifferentiated cells (day 0); lower panels, day 3 of EB differentiation. Fluorescence intensity is indicated on the y axis. The x axis represents FITC, which provides a measure of autofluorescence. (B) Number of BL-CFCs on day 3 EBs for Eng+/-, Eng-/- and -/-:R5 ES cells. Error bars indicate standard deviations from two independent experiments performed in duplicate. *, P<0.01 versus -/-. (C) Representative blast colonies shown at similar magnification (200x).